| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats
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| Autores: |
Moraes,Natália Valadares de
Bergamaschi,Mateus Machado
Bianchi,Maria de Lourdes Pires
Bragheto,Juliana Bordinassi
Malfará,Wilson Roberto
Queiroz,Regina Helena Costa
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| Data: |
2012-03-01
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| Ano: |
2012
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| Palavras-chave: |
Dapsone
Methemoglobinemia
L-arginine
Nitric oxide
L-NAME
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| Resumo: |
Dapsone use is frequently associated to hematological side effects such as methemoglobinemia and hemolytic anemia, which are related to N-hydroxylation mediated by the P450 enzyme system. The aim of the present study was to evaluate the influence of L-arginine supplementation, a precursor for the synthesis of nitric oxide, as single or multiple dose regimens on dapsone-induced methemoglobinemia. Male Wistar rats were treated with L-arginine at 5, 15, 30, 60 and 180 mg/kg doses (p.o., gavage) in single or multiple dose regimens 2 hours prior to dapsone administration (40 mg/kg, i.p.). The effect of the nitric oxide synthase inhibitor L-NAME was investigated by treatment with multiple doses of 30 mg/kg (p.o., gavage) 2 hours before dapsone administration. Blood samples were collected 2 hours after dapsone administration. Erythrocytic methemoglobin levels were assayed by spectrophotometry. The results showed that multiple dose supplementations with 5 and 15 mg/kg L-arginine reduced dapsone-induced methemoglobin levels. This effect is mediated by nitric oxide formation, since the reduction in methemoglobin levels by L-arginine is blocked by simultaneous administration with L-NAME, a nitric oxide synthase inhibitor.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100010
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502012000100010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.48 n.1 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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