| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Fluorometric quantification of protoporphyrin IX in biological skin samples from in vitro penetration/permeation studies
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| Autores: |
Rossetti,Fábia Cristina
Depieri,Lívia Vieira
Tedesco,Antônio Cláudio
Bentley,Maria Vitória Lopes Badra
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| Data: |
2010-12-01
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| Ano: |
2010
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| Palavras-chave: |
Fluorometric quantification
Protoporphyrin IX/determination
Photodynamic therapy
Porcine skin
Cutaneous penetration/in vitro study
Cutaneous permeation/in vitro study
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| Resumo: |
A fluorometric analytical method was developed for quantification of protoporphyrin IX (PpIX) in skin samples and receptor phase solution after in vitro cutaneous penetration/permeation studies. Analytical conditions used were: excitation and emission wavelengths: 400 nm and 632 nm; bandwidth: 0.5 nm; excitation and emission slits: 10/10. PpIX was recovered from two different layers of skin, the stratum corneum (SC) and the epidermis plus dermis ([E+D]), by vortex homogenization, probe and bath sonication, using DMSO as an extraction solvent. The detection and quantification limits were 0.002 and 0.005 μg/mL, respectively. The assay was linear from 0.005 - 0.5 μg/mL. The within-day and between-day assay precision and accuracy in DMSO and receptor phase solution were each studied at the two concentration levels 0.04 and 0.2 μg/mL, and 0.01 and 0.08 μg/mL, respectively. The coefficients of variation and deviation from the theoretical values were lower than 5%. The skin recovery of PpIX from SC and [E+D] layers using two different concentrations (0.5 and 1.0 μg/mL) were all above 90.0%. The method described has potential application to in vitro penetration/permeation studies of PpIX using porcine skin as a biological membrane model.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400017
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502010000400017
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.46 n.4 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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