| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Effect of methionine load on homocysteine levels, lipid peroxidation and DNA damage in rats receiving ethanol
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| Autores: |
Jordao Júnior,Alceu Afonso
Domenici,Fernanda Aparecida
Lataro,Renata Cristina
Portari,Guilherme Vannucchi
Vannucchi,Helio
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| Data: |
2009-12-01
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| Ano: |
2009
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| Palavras-chave: |
Methionine
Homocysteine
Free Radicals
Antioxidants
Ethanol/induced damage
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| Resumo: |
Changes in the metabolism of methionine can cause hyperhomocysteinemia, inducing a triad of atherosclerosis, hypertension, and increased oxidative stress. The generation of free radicals and oxidative damage to DNA is important in the liver damage caused by ethanol. In this study, the effect of methionine overload associated or otherwise with acute administration of ethanol on homocysteine values, damage to DNA, lipoperoxidation and vitamin E was evaluated. Thirty rats were divided into 3 groups: Group Ethanol 24 hours (EG24), Group Methionine 24 hours (MG24), and Group Methionine and Ethanol 24 hours (MEG24). TBARS, vitamin E, GS and, homocysteine values were determined and the Comet assay was carried out. Increased GSH, vitamin E and homocysteine levels were observed for MEG24, and increased TBARS were observed in EG24. The Comet assay showed an increase in DNA damage in EG24 and DNA protection in MEG24. The administration of ethanol decreased antioxidant levels and increased TBARS, indicating the occurrence of oxidative stress with possible DNA damage. The combination of methionine and ethanol had a protective effect against the ethanol-induced damage, but increased the levels of homocysteine.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400014
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502009000400014
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.45 n.4 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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