| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera
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| Autores: |
Utescher,Carla Lilian de Agostini
Buosi,Klariane Laís
Botosso,Viviane Fungaro
Quintilio,Wagner
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| Data: |
2018-01-01
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| Ano: |
2018
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| Palavras-chave: |
Monocytes Activation Test
Pyrogen
Hyperimmune serum
Pyrodetect kit
Cryoblood.
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| Resumo: |
ABSTRACT The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200605
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/s2175-97902018000217530
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.54 n.2 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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