| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles
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| Autores: |
Mattos,Ana Cristina de
Khalil,Najeh Maissar
Mainardes,Rubiana Mara
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| Data: |
2013-03-01
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| Ano: |
2013
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| Palavras-chave: |
Fluorouracil/determination
Nanoparticles
High performance liquid chromatography/quantitative analysis
High performance liquid chromatography/method validation
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| Resumo: |
The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 10.86 and 32.78 ng/mL, respectively. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of 5-FU in polymeric nanoparticles.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100013
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502013000100013
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.49 n.1 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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