| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis
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| Autores: |
Cârcu-Dobrin,Melania
Sabău,Alexandra Georgiana
Hancu,Gabriel
Árpád,Gyéresi
Rusu,Aura
Kelemen,Hajnal
Papp,Lajos Attila
Cârje,Anca
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| Data: |
2020-01-01
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| Ano: |
2020
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| Palavras-chave: |
Amlodipine
Capillary electrophoresis
Chiral separation
Cyclodextrine
Experimental design
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| Resumo: |
The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 ºC, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100530
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/s2175-97902019000318259
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.56 2020
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| Direitos: |
info:eu-repo/semantics/openAccess
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