| Registro completo |
| Provedor de dados: |
Braz. J. Vet. Res. Anim. Sci.
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| País: |
Brazil
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| Título: |
Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae)
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| Autores: |
SALVO,Ligia Maria
MALUCELLI,Maria Ivette Carboni
RICHARTZ,Rosária Regina Tesoni B.
BACILA,Metry
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| Data: |
2000-01-01
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| Ano: |
2000
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| Palavras-chave: |
Liver cells
Cell culture
Cells
Pisces
Metynnis roosevelti
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| Resumo: |
In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000500004
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| Editor: |
Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
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| Relação: |
10.1590/S1413-95962000000500004
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Veterinary Research and Animal Science v.37 n.5 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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