| Registro completo |
| Provedor de dados: |
Scientia Agricola
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| País: |
Brazil
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| Título: |
Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids
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| Autores: |
Ribeiro,Carlos Alexandre Gomes
Pinto,Marcos de Oliveira
Maciel,Talles Eduardo Ferreira
Pastina,Maria Marta
Barros,Everaldo Gonçalves de
Guimarães,Claudia Teixeira
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| Data: |
2017-04-01
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| Ano: |
2017
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| Palavras-chave: |
Zea mays
Fingerprinting
Genotyping
Cultivar protection
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| Resumo: |
ABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163
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| Editor: |
São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
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| Relação: |
10.1590/1678-992x-2016-0079
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| Formato: |
text/html
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| Fonte: |
Scientia Agricola v.74 n.2 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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