| Registro completo |
| Provedor de dados: |
Ciência e Agrotecnologia
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| País: |
Brazil
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| Título: |
In vitro induction of callus from cotyledon and hypocotyl explants of Glycine wightii (Wight & Arn.) Verdc.
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| Autores: |
Silva,André Luis Coelho da
Caruso,Cecília Sulzbacher
Moreira,Renato de Azevedo
Horta,Ana Cecília Góes
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| Data: |
2003-12-01
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| Ano: |
2003
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| Palavras-chave: |
Glycine wightii
Leguminosae
Callogenesis
Tissue culture
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| Resumo: |
With the objective to promote in vitro callus induction, cotyledon and hypocotyl segments of "perennial soybean" (Glycine wightii (Wight & Arn.) Verdc.) were inoculated in basal medium MS supplemented with sucrose (1.5 e 3%) and 0.8% agar and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-furfurylaminopurine (kinetin). The explants were maintained in a dark growth room at 28ºC. The best callus induction was observed in explants (cotyledon and hypocotyl) maintained in medium containing the combination of 2,4-D (1 mg.L-1), kinetin (0.1 mg.L-1) and 3% sucrose. To promote callus subculture, the MS medium was supplemented with different combinations of 2,4-D (0.5 to 4.0 mg.L-1), with or without kinetin (0.1 mg.L-1) and sucrose (1.5 e 3%). The calli were maintained 35 days in a dark growth room at 28ºC. The results indicated that the use of 2,4-D 1.0 mg.L-1 + kinetin 0.1 mg.L-1 + sucrose 3% provided the highest average weight of cotyledons calli fresh matter, whereas the use of 2,4-D 2.0 mg.L-1 + kinetin 0.1 mg.L-1 + sucrose 3% provided the highest average weight of hypocotyl calli fresh matter. High concentrations of 2,4-D, independent of kinetin and sucrose concentrations, promoted oxidation and reduction in fresh weight from calli of cotyledon and hypocotyls.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000600011
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| Editor: |
Editora da Universidade Federal de Lavras
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| Relação: |
10.1590/S1413-70542003000600011
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| Formato: |
text/html
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| Fonte: |
Ciência e Agrotecnologia v.27 n.6 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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