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Provedor de dados:  Ciência e Agrotecnologia
País:  Brazil
Título:  Protoplast production from napier grass and pearl millet triploid hybrids
Autores:  Timbó,Ana Luiza de Oliveira
Davide,Lisete Chamma
Pinto,José Eduardo Brasil Pereira
Pereira,Antônio Vander
Data:  2010-10-01
Ano:  2010
Palavras-chave:  Pennisetum purpureum
Pennisetum glaucum
In vitro cultivation
Interspecific hybrids
Forage
Resumo:  The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020
Editor:  Editora da Universidade Federal de Lavras
Relação:  10.1590/S1413-70542010000500020
Formato:  text/html
Fonte:  Ciência e Agrotecnologia v.34 n.5 2010
Direitos:  info:eu-repo/semantics/openAccess
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