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Provedor de dados:  Ciência e Agrotecnologia
País:  Brazil
Título:  Molecular characterization of elephantgrass accessions through RAPD markers
Autores:  Passos,Leônidas Paixão
Machado,Marco Antonio
Vidigal,Maria Coletta
Campos,Ana Lúcia
Data:  2005-06-01
Ano:  2005
Palavras-chave:  Molecular markers
Elephantgrass
RAPD
DNA
Resumo:  Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix, which was utilized in a UPGMA grouping analysis. Elephantgrass cultivars Cameroon and Vruckwona were the accessions mostly divergent from the others, with an average genetic distance of 0.34. The accessions with the lowest average genetic distances from the others were Pioneiro and CNPGL 27-5, both with a distance of 0.25. Overall, genetic distances ranged from 0.06 to 0.43, indicating little genetic variability for the set of accessions, despite the contrasting morphology of the studied genotypes.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300009
Editor:  Editora da Universidade Federal de Lavras
Relação:  10.1590/S1413-70542005000300009
Formato:  text/html
Fonte:  Ciência e Agrotecnologia v.29 n.3 2005
Direitos:  info:eu-repo/semantics/openAccess
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