Registro completo |
Provedor de dados: |
Ciência Rural
|
País: |
Brazil
|
Título: |
Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
|
Autores: |
Busso,Cleverson
Kaneshima,Edilson Nobuyoshi
Franco,Francisco de Assis
Querol,Carmen Boto
Castro-Prado,Marialba Avezum Alves de
|
Data: |
2007-12-01
|
Ano: |
2007
|
Palavras-chave: |
Fusarium graminearum
Genetic variability
RAPD
Vegetative compatibility groups
|
Resumo: |
Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.
|
Tipo: |
Info:eu-repo/semantics/article
|
Idioma: |
Inglês
|
Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050
|
Editor: |
Universidade Federal de Santa Maria
|
Relação: |
10.1590/S0103-84782007000600050
|
Formato: |
text/html
|
Fonte: |
Ciência Rural v.37 n.6 2007
|
Direitos: |
info:eu-repo/semantics/openAccess
|