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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Honeybee venom: influence of collection on quality and cytotoxicity
Autores:  Abrantes,Allyson Fortunato
Rocha,Thayse Cavalcante da
Lima,Amanda Beatriz Sales de
Cavalcanti,Mônica Tejo
Data:  2017-01-01
Ano:  2017
Palavras-chave:  Bees
Beekeeping
Bee venom
Artemia salina L
Resumo:  ABSTRACT: Apitoxin is the venom produced by bees. It is a complex chemical compound, rich in protein substances and with pharmacological effects. This study was carried out with the objective of comparing the quality of apitoxin extracted in an apiary in different parts of the collector in relation to moisture content, protein analysis and cytotoxicity assay with Artemia salina L. Type 1 apitoxin was collected from glass slabs at the entrance to the hive, while type 2 apitoxin was collected from the waste accumulated in the collection rods and treated by rinsing in distilled water. Both apitoxins presented significant differences (P>0.05) in relation to protein profile, with type 1 showing a higher content (77.8%) than type 2 (51.9%), and presented polypeptide bands with more than 50% of their nitrogenous components having molecular weight below 10KDa. Regarding cytotoxicity assays, type 1 apitoxin had LD50 of 71.5μg mL-1, while type 2 had LD50 of 191.6μg mL-1. Thus, the region where apitoxin accumulates in the collector does influence the product quality if moisture and protein contents are in accordance with the standards recommended in specific legislation, and so it can be commercialized by the beekeeper.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000401
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/0103-8478cr20160486
Formato:  text/html
Fonte:  Ciência Rural v.47 n.10 2017
Direitos:  info:eu-repo/semantics/openAccess
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