| Registro completo |
| Provedor de dados: |
Ciência Rural
|
| País: |
Brazil
|
| Título: |
Genotyping of South American clinical isolates of Pythium insidiosum based on single nucleotide polymorphism-based multiplex PCR
|
| Autores: |
Weiblen,Carla
Azevedo,Maria Isabel de
Ianiski,Lara Baccarin
Stibbe,Paula Cristina
Pereira,Daniela Isabel Brayer
Zanette,Régis Adriel
Sangioni,Luís Antônio
Rivero,Rodolfo
Santurio,Janio Morais
Botton,Sônia de Avila
|
| Data: |
2019-01-01
|
| Ano: |
2019
|
| Palavras-chave: |
Pythium insidiosum
Pythiosis
Molecular detection
Genotype
Single nucleotide polimorphisms
|
| Resumo: |
ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II, and the Thai isolate to clade III. P. aphanidermatum, P.torulosum, P.rhizo-oryzae and P.pachycaule voucher isolates were not amplified. For the first time, a P. insidiosum isolate from Uruguay, South America, was included in molecular analyzes. By SNP-based multiplex-PCR, it was possible to perform the identification and genotyping of the South American isolates of P. insidiosum, demonstrating similar genetic characteristics of these isolates.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450
|
| Editor: |
Universidade Federal de Santa Maria
|
| Relação: |
10.1590/0103-8478cr20180744
|
| Formato: |
text/html
|
| Fonte: |
Ciência Rural v.49 n.1 2019
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
|
