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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures
Autores:  Rosato,Paula Nunes
Gama,Fernanda Gomes Velasque
Santana,Aureo Evangelista
Data:  2006-12-01
Ano:  2006
Palavras-chave:  Cerebrospinal fluid
Resumo:  Disorders involving the nervous system have a great importance in veterinary medicine because they have a high incidence and few auxiliary tools for their diagnostic, prognostic and evaluation of the employed therapy. Today, the diagnostic is based, mainly, on the patient history and neurologic examination. Hence, evaluation of the cerebrospinal fluid elements is one of the few alternatives to clinically access the central nervous system (CNS). Even with the great usefullness of the physical-chemical and cytoscopy exams of the CSF in veterinary neurology, there are few studies concerning the stability of its elements under freezing storage. The present study was aimed at verifing the influence of temperature and period of conservation on physical-chemical characteristics of the CSF of healthy dogs. For that purpose, CSF samples were collected by puncture of the cisterna magna of dogs clinically healthy, and then were analysed for density, pH, glucorrhachia, total proteins and activity of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST), after storage in different temperatures (25°C, 4°C and -4°C) and periods (immediately after collection and after 24 hours, 48 hours, a week and a month). Regarding the obtained results it was possible to verify that the parameters studied were stable up to a month of storage, under freezing at -4°C.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/S0103-84782006000600021
Formato:  text/html
Fonte:  Ciência Rural v.36 n.6 2006
Direitos:  info:eu-repo/semantics/openAccess

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