| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Virulence factors and phylotyping of Escherichia coli isolated from non-diarrheic and diarrheic water buffalo calves
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| Autores: |
Coura,Fernanda Morcatti
Diniz,Soraia de Araújo
Silva,Marcos Xavier
Oliveira,Cairo Henrique Sousa de
Mussi,Jamili Maria Suhet
Oliveira,Camila Stefanie Fonseca de
Lage,Andrey Pereira
Heinemann,Marcos Bryan
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| Data: |
2019-01-01
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| Ano: |
2019
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| Palavras-chave: |
Buffalo calves
Diarrhea
Escherichia coli
Pathovars
Phylogenetic group
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| Resumo: |
ABSTRACT: This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in non-diarrheic animals were NTEC (21/50), STEC (17/50), EHEC (1/50) and EAEC (7/50). E. coli strains positive for EAST-1 (P=0.008) and phylogroup C (P = 0.05) were associated with the presence of diarrhea. Phylogenetic analysis showed that 58.95% of the isolates belonged to phylogroup B1, followed by E (9.70%), B2 (5.90%), C (5.90%), D (5.22%), A (2.24%), and F (1.50%). Phylogroup B1 predominated in pathogenic E. coli isolated from water buffalo, and phylogroup C constituted an enteropathogenic E. coli for water buffalo calves.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20180998
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.49 n.5 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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