| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats
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| Autores: |
Baumworcel,Natasha
Pereira,Joylson de Jesus
Soares,Ana Maria Barros
Souza,Guilherme Nunes
Almosny,Nadia Regina Pereira
Castro,Tatiana Xavier de
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| Data: |
2019-01-01
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| Ano: |
2019
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| Palavras-chave: |
FHV-1
Conjunctivitis
Shelter medicine
QPCR
Animal shelter
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| Resumo: |
ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20190067
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.49 n.5 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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