| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Micropropagation of two species of foliage anthurium by direct organogenesis
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| Autores: |
Campos,Arlene Santisteban
Carvalho,Ana Cristina Portugal Pinto de
Castro,Ana Cecília Ribeiro de
Bertini,Cândida Hermínia Campos de Magalhães
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| Data: |
2018-01-01
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| Ano: |
2018
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| Palavras-chave: |
Anthurium bonplandii
Anthurium maricense
6-BA
Tissue culture.
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| Resumo: |
ABSTRACT: The objective of the present research was to develop a protocol for micropropagation of Anthurium bonplandii and Anthurium maricense by direct organogenesis. Nodal segments, with two or three nodes, were used as explants. The cultures were kept in a growth chamber at a temperature of 25±2ºC, under a photoperiod of 16 hours and a luminosity of 30μmol m-2 s-1. At 60 days, the number of regenerated buds per explant (NBE) was counted. The experiment was carried out in an entirely randomised design consisting of six treatments for six different concentrations of 6-benzylaminopurine (6-BA) added to the P2 (Pierik) medium (0.0, 1.11, 2.22, 3.33, 4.44, and 5.55µM). All the treatments were performed in four repetitions with 10 culture tubes containing one explant each. The regression analyses were adjusted to a quadratic model, with R2 = 88.7% and 62.4% for A. maricense and A. bonplandii, respectively. The regressions indicate that the addition of 6-BA to the P2 medium resulted in larger values of NBE in both the species. The ideal 6-BA concentration for micropropagation varied depending on the species, with 2.5 and 1.7 NBE determined at 6-BA concentrations of 4.70 and 3.37µM for A. maricense and A. bonplandii, respectively.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700401
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/0103-8478cr20170817
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.48 n.7 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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