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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
Autores:  Fante,Camila Argenta
Goulart,Patrícia de Fátima Pereira
Alves,José Donizeti
Henrique,Paôla de Castro
Fries,Daniela Deitos
Data:  2011-12-01
Ano:  2011
Palavras-chave:  Glycine max
Hypoxia
Functional food
Resumo:  The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200029
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/S0103-84782011001200029
Formato:  text/html
Fonte:  Ciência Rural v.41 n.12 2011
Direitos:  info:eu-repo/semantics/openAccess
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