| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A
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| Autores: |
Ferreira,Helena Lage
Spilki,Fernando Rosado
Santos,Márcia Mercês Aparecida Bianchi dos
Almeida,Renata Servan de
Arns,Clarice Weis
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| Data: |
2009-08-01
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| Ano: |
2009
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| Palavras-chave: |
Avian metapneumovirus G
F
N genes real time RT-PCR RT-PCR
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| Resumo: |
Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an established test for the attachment (G) gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F- based RRT-PCR and F-based conventional RT-PCR (10(0.3) to 10¹ TCID50 mL-1). The present study suggests that the conventional F-based RT-PCR presented similar detection limit when compared to N- and F-based RRT-PCR and they can be successfully used for AMPV/A detection.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000500022
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84782009005000057
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.39 n.5 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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