| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
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| País: |
Chile
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| Título: |
A preferable approach to clone hLIF cDNA from the genomic DNA
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| Autores: |
Cui,Yihong
Zhu,Guangqin
Chen,Qiuju
Wang,Yunfei
Yang,Mingming
Song,Yuxuan
Wang,Jiangang
Cao,Binyun
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| Data: |
2011-05-01
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| Ano: |
2011
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| Palavras-chave: |
CDNA cloning
HLIF
Oral mucous membrane
Overlap PCR
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| Resumo: |
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012
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| Editor: |
Pontificia Universidad Católica de Valparaíso
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| Formato: |
text/html
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| Fonte: |
Electronic Journal of Biotechnology v.14 n.3 2011
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