| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
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| País: |
Chile
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| Título: |
Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
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| Autores: |
Rasmussen-Poblete,Susana
Valdes,Jorge
Gamboa,Maria Cecilia
Valenzuela,Pablo D.T
Krauskopf,Erwin
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| Data: |
2008-04-01
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| Ano: |
2008
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| Palavras-chave: |
Cellulose
Cold-temperature
EST database
Forest biotechnology
Lignin
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| Resumo: |
Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008
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| Editor: |
Pontificia Universidad Católica de Valparaíso
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| Formato: |
text/html
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| Fonte: |
Electronic Journal of Biotechnology v.11 n.2 2008
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