| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
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| País: |
Chile
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| Título: |
Callus induction and plant regeneration of Ulex europaeus
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| Autores: |
Ramírez,Ingrid
Dorta,Fernando
Cuadros-Inostroza,Álvaro
Peña-Cortés,Hugo
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| Data: |
2012-07-01
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| Ano: |
2012
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| Palavras-chave: |
Antioxidants
Callus induction
Fabaceae
Gorse
Plant regeneration
Ulex europaeus
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| Resumo: |
A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400007
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| Editor: |
Pontificia Universidad Católica de Valparaíso
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| Formato: |
text/html
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| Fonte: |
Electronic Journal of Biotechnology v.15 n.4 2012
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