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Provedor de dados:  Electron. J. Biotechnol.
País:  Chile
Título:  Expression of RNA polymerase IV and V in Oryza sativa
Autores:  Moo,Leydi de Rocio Canche
González,Angela Kú
Rodríguez-Zapata,Luis Carlos
Suarez,Victor
Castaño,Enrique
Data:  2012-03-01
Ano:  2012
Palavras-chave:  Oryza sativa
RNA pol IV
RNA pol V
Somatic embryogenesis
Zygotic embryogenesis
Resumo:  RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.
Tipo:  Journal article
Idioma:  Inglês
Identificador:  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009
Editor:  Pontificia Universidad Católica de Valparaíso
Formato:  text/html
Fonte:  Electronic Journal of Biotechnology v.15 n.2 2012
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