| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
|
| País: |
Chile
|
| Título: |
Isolation of high quality RNA from Polyporus umbellatus (Pers.) Fries
|
| Autores: |
Zhang,Yuejin
Qin,Yali
Guo,Lijun
Zhou,Zili
Liang,Zongsuo
Zhang,Chenlu
Guo,Hongbo
|
| Data: |
2012-09-01
|
| Ano: |
2012
|
| Palavras-chave: |
Chuling
Medicinal fungus
Polyporus umbellatus
RNA isolation
|
| Resumo: |
Background: The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality RNA applicable for sequence-related amplification polymorphism (SRAP) PCR and GDP-D-mannose pyrophosphorylase (GMP) gene expression profiles. Both A260/A280 and A260/A230 ratios of the best Trizol Plus + RNAiso-mate for Plant Tissue method are around 2 with a yield of 1122.00 ± 0.21 ng μl-1. The Trizol method also showed good quality with the yield 469.60 ng μl-1. The SRAP PCR amplified clear and polymorphic bands in all five cDNA samples transcribed from RNA by using primer Me4-Em4. GMP gene fragment (1251 bp) was successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. Conclusion: All these results showed that the total RNA isolated by this protocol is of sufficient quality for subsequent molecular applications.
|
| Tipo: |
Journal article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500010
|
| Editor: |
Pontificia Universidad Católica de Valparaíso
|
| Formato: |
text/html
|
| Fonte: |
Electronic Journal of Biotechnology v.15 n.5 2012
|
