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Provedor de dados:  Electron. J. Biotechnol.
País:  Chile
Título:  Selection of polyvalent bacteriophages infecting Salmonella entérica serovar Choleraesuis
Autores:  Parra,Bárbara
Data:  2016-05-01
Ano:  2016
Palavras-chave:  Bacteriophage
Salmonella enterica serovar Choleraesuis
Resumo:  Background: Ideally, bacteriophages of pathogenic bacterial hosts should be polyvalent to be able to replicate in an alternative nonpathogenic bacterium. Thus, accidental infection by the original host can be avoided when bacteriophage lysates are used in biocontrol protocols. Results: From 15 wastewater samples, collected at different sites in the V Region in Chile, we selected three bacteriophages (FC, FP, and FQ) capable of productively infecting Salmonella enterica serovar Choleraesuis. By transmission electron microscopy (TEM) observation, the bacteriophages were found to belong to the order Caudoviridae. Molecular analyses indicated that FC, FP, and FQ contained double-stranded DNA genomes, of sizes similar to bacteriophage P22, and distinct recognition sites for the restriction endonucleases HaeIII and HindIII. Assays of host range revealed that the bacteriophages were polyvalent and thus capable of infecting different strains of Escherichia coli and other serovars of Salmonella. Conclusion: We have isolated new bacteriophages of the serovar Choleraesuis with various potential applications in relation to this pathogenic bacterium.
Tipo:  Journal article
Idioma:  Inglês
Editor:  Pontificia Universidad Católica de Valparaíso
Formato:  text/html
Fonte:  Electronic Journal of Biotechnology v.19 n.3 2016

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