| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
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| Autores: |
Pinheiro,Fábio
Palma-Silva,Clarisse
Barros,Fábio de
Cozzolino,Salvatore
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| Data: |
2009-01-01
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| Ano: |
2009
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| Palavras-chave: |
Epidendrum
Orchidaceae
Short tandem repeat
Cross-amplification
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| Resumo: |
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200021
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572009005000037
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.32 n.2 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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