| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Quantitative real-time PCR for the clinical detection of Helicobacter pylori
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| Autores: |
Ribeiro,Marcelo Lima
Ecclissato,Christina Cunha
Mattos,Ricardo Gabriel
Mendonca,Sergio
Pedrazzoli Jr.,José
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| Data: |
2007-03-01
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| Ano: |
2007
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| Palavras-chave: |
Diagnostic methods
Helicobacter pylori
Real-time PCR
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| Resumo: |
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according to the references methods, 16 were H. pylori-positive by PCR, 10 of which were patients who had received H. pylori eradication therapy and 6 were untreated patients. Based on these findings we recommend that RT-PCR should be use in addition to standard methods in clinical studies to monitor the results of H. pylori eradication therapy.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572007000300022
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.30 n.2 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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