| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Quantification of bovine cytokine gene expression using real-time RT-PCR methodology
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| Autores: |
Zaros,Lilian Giotto
Bricarello,Patrízia Ana
Amarante,Alessandro Francisco Talamini
Coutinho,Luiz Lehmann
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| Data: |
2007-01-01
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| Ano: |
2007
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| Palavras-chave: |
Cytokine gene expression
Real-time RT-PCR
Cattle
Gastrointestinal nematodes
Bovine cytokines
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| Resumo: |
T cells produce cytokines that affect host response to infection. This paper reports real-time RT-PCR conditions and validation steps for accurate quantification of Bos indicus cytokines, interleukin (IL)-2, IL-4, IL-8, IL12p-35, IL-13, tumoral necrosis factor (TNF)-alpha, interferon (IFN)-gamma, monocyte chemoattractant proteins (MCP)-1 and MCP-2, and the glycoprotein mucin (MUC)-1 in two groups of Nelore cattle, one resistant and the other susceptible to gastrointestinal nematode infections. RPL-19 was shown to be an ideal internal control gene, since its expression was constant across treatments and presented lower variation when compared to the GAPDH gene. The optimized conditions established in the present study can be used to determine the immune response of cattle under different experimental conditions, such as viral, bacterial and parasite infections.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400012
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572007000400012
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.30 n.3 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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