| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
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| Autores: |
Du,Yue-feng
Liang,Liang
Shi,Ying
Long,Qing-zhi
Zeng,Jin
Wang,Xin-yang
He,Da-lin
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| Data: |
2012-01-01
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| Ano: |
2012
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| Palavras-chave: |
Prostate cancer
DNA methylation
DNMT3
RNA interference
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| Resumo: |
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572012005000021
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.35 n.1 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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