| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Use of chromosome microdissection in fish molecular cytogenetics
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| Autores: |
Henning,Frederico
Trifonov,Vladimir
Almeida-Toledo,Lurdes Foresti de
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| Data: |
2008-01-01
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| Ano: |
2008
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| Palavras-chave: |
Fluorescence in situ hybridization
Chromosome painting
Sex chromosomes
Cross-FISH
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| Resumo: |
Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572008000200022
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.31 n.1 suppl.0 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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