| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
|
| País: |
Brazil
|
| Título: |
Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases
|
| Autores: |
Nerys-Junior,Arildo
Costa,Lendel C.
Braga-Dias,Luciene P.
Oliveira,Márcia
Rossi,Átila D.
Cunha,Rodrigo Delvecchio da
Gonçalves,Gabriel S.
Tanuri,Amilcar
|
| Data: |
2014-01-01
|
| Ano: |
2014
|
| Palavras-chave: |
CCR5
Genome editing
Gene knockout
TALEN
HMA
|
| Resumo: |
Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double- strand breaks that allow gene disruption or gene insertion, thereby facilitating genetic manipulation. The major problem associated with this approach is the labor-intensive procedures required to screen and confirm the cellular modification by nucleases. In this work, we produced a TALEN that targets the human CCR5 gene and developed a heteroduplex mobility assay for HEK 293T cells to select positive colonies for sequencing. This approach provides a useful tool for the quick detection and easy assessment of nuclease activity.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100018
|
| Editor: |
Sociedade Brasileira de Genética
|
| Relação: |
10.1590/S1415-47572014000100018
|
| Formato: |
text/html
|
| Fonte: |
Genetics and Molecular Biology v.37 n.1 2014
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
