| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Use of the TSPY gene for sexing cattle
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| Autores: |
Lemos,Daniela Cristina
Rios,Álvaro Fabrício Lopes
Caetano,Lisandra Cristina
Lôbo,Raysildo Barbosa
Vila,Reginaldo Aparecido
Martelli,Lúcia
Takeuchi,Paula Lumy
Ramos,Ester Silveira
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| Data: |
2005-03-01
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| Ano: |
2005
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| Palavras-chave: |
Bovine
Cattle
PCR
Sexing
TSPY
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| Resumo: |
The Y-encoded, testis-specific protein (TSPY) is a Y-specific gene. The copy numbers of TSPY range from 20 to 60 in men and up to 200 in bulls. In this study, we examined the possibility of using the TSPY gene to sex cattle. DNA from blood samples of 100 Nelore cattle (50 males and 50 females) from the Nelore Cattle Breeding Program (PMGRN) was screened for TSPY by PCR using TSPY-specific primers. The assay was highly specific since all male samples were TSPY-positive and all female samples were negative. Positive results were also obtained at low DNA concentrations (less than 1 rhog/muL). These results showed that TSPY was a good male-specific marker, the usefulness of which was enhanced by the high copy number of the gene. This is the first report to demonstrate the applicability of TSPY for sexing cattle.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100020
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572005000100020
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.28 n.1 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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