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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  A new approach for obtaining rapid uniformity in rice (Oryza sativa L.) via a 3x x 2x cross
Autores:  Xing,Shaochen
Data:  2010-01-01
Ano:  2010
Palavras-chave:  F-test
SSR marker
Resumo:  A triploid (2n = 3x = 36) rice plant was obtained by screening a twin seedling population in which each seed germinated to two or three sprouts that were then crossed with diploid plants. One diploid plant was chosen among the various F1 progenies and developed into an F2 population via self-pollination. Compared with the control variety Shanyou 63, this F2 population had a stable agronomical performance in field trials, as confirmed by the F-test. The stability of the F2 population was further substantiated by molecular analysis with simple sequence repeat markers. Specifically, of 160 markers assayed, 37 (covering all 12 chromosomes) were polymorphic between the parental lines. Testing the F1 hybrid individually with these markers showed that each PCR product had only a single band instead of two bands from each parent. The bands were identical to either maternal (23 markers) or paternal (eight markers) bands or distinct from both parents (six markers). The amplified bands of all 60 randomly selected F2 plants were uniform and identical to those of the F1 hybrid. These results suggest that the F1 plant is a non-segregating hybrid and that a stable F2 population was obtained. This novel system provides an efficient means for shortening the cycle of hybrid rice seed production.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572010005000023
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.33 n.2 2010
Direitos:  info:eu-repo/semantics/openAccess

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