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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Annexin II mRNA expression in bovine oocytes during follicular development
Autores:  Costa,Luis Fabiano Santos da
Machado,Márcia Silveira Netto
Oliveira,João Francisco Coelho de
Zamberlan,Gustavo
Gonçalves,Paulo Bayard Dias
Data:  2006-01-01
Ano:  2006
Palavras-chave:  Bovine oocytes
Annexin II
Retinol
Resumo:  We investigated the expression of calcium-dependent phospholipid binding protein annexin-II (Ann-II) messenger RNA (mRNA) during preantral follicle development and in oocytes from antral follicles of different diameters (< 3 mm, 5 to 8 mm and > 8 mm). The action of retinol on Ann-II mRNA expression in mature oocytes was also examined. Only oocytes from secondary preantral follicles expressed Ann-II mRNA and at the germinal vesicle stage expression by oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) compared with oocytes from follicles smaller than 3 mm or between 5 and 8 mm. Ann-II mRNA expression by metaphase II oocytes from follicles larger than 8 mm was significantly higher (p < 0.05) than that from oocytes from follicles smaller than 3 mm, with oocytes from both these size-classes showing similar levels of Ann-II mRNA expression as oocytes recovered from 5-8 mm follicles. In the presence of retinol, Ann-II mRNA expression was higher than when retinol was absent (p < 0.05). Our data indicate that Ann-II mRNA expression is highest in competent oocytes and that retinol increases Ann-II mRNA and may be involved in the regulation of oocyte competence by decreasing the translation and/or degradation of Ann-II mRNA.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200031
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572006000200031
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.29 n.2 2006
Direitos:  info:eu-repo/semantics/openAccess
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