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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine)
Autores:  Schlögl,Paulo Sérgio
Santos,André Luis Wendt dos
Vieira,Leila do Nascimento
Floh,Eny Iochevet Segal
Guerra,Miguel Pedro
Data:  2012-01-01
Ano:  2012
Palavras-chave:  Seed development
Brazilian Pine
Embryogenesis-regulating genes
Zygotic embryogenesis
Resumo:  Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz.,ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semiquantitative RT-PCR during embryo development and germination. All the eight were found to be differentially expressed in the various developmental stages of zygotic embryos, seeds and seedling tissues. To our knowledge, this is the first report on embryogenesis-regulating gene expression in members of the Araucariaceae family, as well as in plants with recalcitrant seeds.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100025
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572012005000005
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.35 n.1 2012
Direitos:  info:eu-repo/semantics/openAccess
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