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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)
Autores:  Costa,Laiana Cristina da
Azeredo-Oliveira,Maria Tercília Vilela de
Tartarotti,Ester
Data:  2008-01-01
Ano:  2008
Palavras-chave:  Triatoma klugi
Triatomine
Cytogenetics
Nucleolus
Karyotype
Meiosis
Resumo:  Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572008000300008
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.31 n.2 2008
Direitos:  info:eu-repo/semantics/openAccess
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