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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
Autores:  Silva,Guilherme Messias da
Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
Data:  2009-01-01
Ano:  2009
Palavras-chave:  Chromosome rearrangements
Heterochromatin
Karyotype
NORs
FISH
Resumo:  Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572009005000040
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.32 n.2 2009
Direitos:  info:eu-repo/semantics/openAccess
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