| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
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| Autores: |
Pereira,Wendell Jacinto
Melo,Arthur Tavares de Oliveira
Coelho,Alexandre Siqueira Guedes
Rodrigues,Fabiana Aparecida
Mamidi,Sujan
Alencar,Sérgio Amorim de
Lanna,Anna Cristina
Valdisser,Paula Arielle Mendes Ribeiro
Brondani,Claudio
Nascimento-Júnior,Ivanildo Ramalho do
Borba,Tereza Cristina de Oliveira
Vianello,Rosana Pereira
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| Data: |
2020-01-01
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| Ano: |
2020
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| Palavras-chave: |
Phaseolus vulgaris
Water deficit
Functional genomics
Single nucleotide polymorphism (SNP)
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| Resumo: |
Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/1678-4685-gmb-2018-0259
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.43 n.1 2020
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| Direitos: |
info:eu-repo/semantics/openAccess
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