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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Genotoxic evaluation of an industrial effluent from an oil refinery using plant and animal bioassays
Autores:  Rodrigues,Fernando Postalli
Angeli,José Pedro Friedmann
Mantovani,Mário Sérgio
Guedes,Carmen Luisa Barbosa
Jordão,Berenice Quinzani
Data:  2010-01-01
Ano:  2010
Palavras-chave:  Allium cepa
HTC cells
Micronucleus
Comet assay
Petroleum waste disposal
Resumo:  Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types, plant (Allium cepa) and mammal (HTC) cells, for more accurately assessing genotoxicity in environmental samples.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100028
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572010005000006
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.33 n.1 2010
Direitos:  info:eu-repo/semantics/openAccess
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