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Provedor de dados:  Horticultura Brasileira
País:  Brazil
Título:  Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
Autores:  Pontes,Nadson C
Fujinawa,Miriam F
Oliveira,José R
Data:  2017-03-01
Ano:  2017
Palavras-chave:  Bacterial wilt
Culture medium
Diagnosis
SMSA-E.
Resumo:  ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103 cfu/gram of soil. SMSA-E (Selective Medium South Africa - Elphinstone) was found to be the most effective in suppressing the growth of contaminating microorganisms, resulting in the lowest rate of repression of R. solanacearum and the highest recovery rate of the bacterium in the soil.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041
Editor:  Associação Brasileira de Horticultura
Relação:  10.1590/s0102-053620170107
Formato:  text/html
Fonte:  Horticultura Brasileira v.35 n.1 2017
Direitos:  info:eu-repo/semantics/openAccess
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