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Provedor de dados:  Horticultura Brasileira
País:  Brazil
Título:  In vitro propagation of five Alocasia species
Autores:  Bhatt,Arvind
Stanly,Christine
Keng,Chan Lai
Data:  2013-06-01
Ano:  2013
Palavras-chave:  Alocasia amazonica
A. cuprea
A. robusta
A. longiloba
A. chaii
Culture medium
Acclimatization
Resumo:  The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot growth and proliferation for all the tested species. For A. amazonica, A. cuprea, A. robusta and A. longiloba, shake flask system using liquid medium of the same constituents stimulated more shoot proliferation as compared to agar-gelled medium. However, for A. chaii there was no significant difference. All the in vitro plantlets with well developed roots and leaves were successfully acclimatized with more than 90% survival rate.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006
Editor:  Associação Brasileira de Horticultura
Relação:  10.1590/S0102-05362013000200006
Formato:  text/html
Fonte:  Horticultura Brasileira v.31 n.2 2013
Direitos:  info:eu-repo/semantics/openAccess
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