| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins
|
| País: |
Brazil
|
| Título: |
In vitro assay of biological and chemical toxins using antibodies against lethal toxin neutralizing factor
|
| Autores: |
LIPPS,B. V.
|
| Data: |
2002-01-01
|
| Ano: |
2002
|
| Palavras-chave: |
Antibodies
In vitro assay
Lethal toxin neutralizing factor
Toxins
Venoms
|
| Resumo: |
Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently, mouse bioassay is in use for toxin detection and assay. Binding affinity of IgG from anti-LT-10 showed a linear relationship with mouse bioassay by ELISA detection limit to some toxins only. This may be due to the fact that anti-LTNF and anti-LT-10 detected the toxins that were not lethal to mouse. Thus, anti-LTNF and anti-LT-10 can be useful in assaying toxins as an alternative to mouse bioassay.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200003
|
| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
|
| Relação: |
10.1590/S0104-79302002000200003
|
| Formato: |
text/html
|
| Fonte: |
Journal of Venomous Animals and Toxins v.8 n.2 2002
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
