| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins incl. Trop. Dis.
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| País: |
Brazil
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| Título: |
Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
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| Autores: |
Carnieli JR,P.
Ventura,A. M.
Durigon,E. L.
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| Data: |
2007-01-01
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| Ano: |
2007
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| Palavras-chave: |
Rabies
G gene
Plasmids
Transfection
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| Resumo: |
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400015
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| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos
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| Relação: |
10.1590/S1678-91992007000400015
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| Formato: |
text/html
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| Fonte: |
Journal of Venomous Animals and Toxins including Tropical Diseases v.13 n.4 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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