| Registro completo |
| Provedor de dados: |
Neotropical Ichthyology
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| País: |
Brazil
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| Título: |
Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
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| Autores: |
Souza-Shibatta,Lenice
Ferreira,Dhiego Gomes
Oliveira,Claudio
Almeida,Fernanda Simões de
Shibatta,Oscar Akio
Sofia,Silvia Helena
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| Data: |
2013-09-01
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| Ano: |
2013
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| Palavras-chave: |
Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
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| Resumo: |
Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581
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| Editor: |
Sociedade Brasileira de Ictiologia
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| Relação: |
10.1590/S1679-62252013000300011
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| Formato: |
text/html
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| Fonte: |
Neotropical Ichthyology v.11 n.3 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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