| Registro completo |
| Provedor de dados: |
Phyton
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| País: |
Argentina
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| Título: |
Cloning and expression analysis of rubredoxin from cold-treated banana leaves
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| Autores: |
Feng¹,RJ
Lu,LF
Yuan,KH
Cheng,P
Zhang,LL
Qi,JF
Ren,Y
Xu,XL
Zhang,XB
Zhou,LY
Zhang,YD
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| Data: |
2010-12-01
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| Ano: |
2010
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| Palavras-chave: |
Rubredoxin
Expression
Banana
Stress response
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| Resumo: |
A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of MaRd1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of MaRd1 transcript accumulation in leaves. This finding indicated a role of MaRd1 in the response to these abiotic stresses.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200008
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| Editor: |
Fundación Rómulo Raggio
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| Formato: |
text/html
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| Fonte: |
Phyton (Buenos Aires) v.79 n.2 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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