| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
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| Autores: |
Silva,Tatiane Loureiro da
Scherwinski-Pereira,Jonny Everson
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| Data: |
2011-04-01
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| Ano: |
2011
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| Palavras-chave: |
Ex situ conservation
Germplasm bank
Micropropagation
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| Resumo: |
The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400007
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2011000400007
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.46 n.4 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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