| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration
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| Autores: |
Droste,Annette
Silva,Anelise Machado da
Souza,Iliane Freitas de
Wiebke-Strohm,Beatriz
Bücker-Neto,Lauro
Bencke,Marta
Sauner,Márcia Viviane
Bodanese-Zanettini,Maria Helena
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| Data: |
2010-07-01
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| Ano: |
2010
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| Palavras-chave: |
Glycine max
Brazilian cultivars
Embryogenic potential
Genetic engineering
Soybean transformation
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| Resumo: |
The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated charcoal to maturation medium increased embryo differentiation rates, which resulted in a higher number of regenerated plants. The BRSMG 68 Vencedora cultivar was found to have a high potential for embryo induction, embryo proliferation and plant regeneration. The potential of this cultivar for somatic embryogenesis was similar to that observed for cultivar IAS-5, which is currently used for soybean transformation in Brazil. BRSMG 68 Vencedora may be a good alternative genotype for soybean genetic engineering via somatic embryogenesis protocols.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700012
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2010000700012
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.45 n.7 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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