| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Plant regeneration from protoplast of Brazilian citrus cultivars
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| Autores: |
GLORIA,FERNANDA JANUZZI MENDES DA
MOURÃO FILHO,FRANCISCO DE ASSIS ALVES
MENDES,BEATRIZ MADALENA JANUZZI
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| Data: |
2000-04-01
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| Ano: |
2000
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| Palavras-chave: |
Cultivar improvement
Polyethylene glycol
Rootstock
Tissue culture
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| Resumo: |
A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract. Well defined embryos were germinated in modified MT medium with addition of GA3 (2.0 muM) and malt extract (500 mg/L). Plant regeneration was also achieved by adventitious shoots obtained through direct organogenesis of not well defined embryos in modified MT medium with addition of malt extract (500 mg/L), BAP (1.32 muM), NAA (1.07 muM) and coconut water (10 mL/L). Plantlets were transferred to root medium. Rooted plants were transferred to a greenhouse for further adaptation and development.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400008
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2000000400008
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.35 n.4 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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