| Registro completo |
| Provedor de dados: |
PAB
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| País: |
Brazil
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| Título: |
Optimal use of SSR markers for varietal identification of upland cotton
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| Autores: |
Pereira,Guilherme da Silva
Cazé,Ana Luíza Ramos
Silva,Michelle Garcia da
Almeida,Vanessa Cavalcante
Magalhães,Fernanda Oliveira da Cunha
Silva Filho,João Luís da
Barroso,Paulo Augusto Vianna
Hoffmann,Lúcia Vieira
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| Data: |
2015-07-01
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| Ano: |
2015
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| Palavras-chave: |
Gossypium hirsutum
Cultivar discrimination
Intraspecific polymorphism
Microsatellite
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| Resumo: |
Abstract: The objective of this work was to identify polymorphic simple sequence repeat (SSR) markers for varietal identification of cotton and evaluation of the genetic distance among the varieties. Initially, 92 SSR markers were genotyped in 20 Brazilian cotton cultivars. Of this total, 38 loci were polymorphic, two of which were amplified by one primer pair; the mean number of alleles per locus was 2.2. The values of polymorphic information content (PIC) and discrimination power (DP) were, on average, 0.374 and 0.433, respectively. The mean genetic distance was 0.397 (minimum of 0.092 and maximum of 0.641). A panel of 96 varieties originating from different regions of the world was assessed by 21 polymorphic loci derived from 17 selected primer pairs. Among these varieties, the mean genetic distance was 0.387 (minimum of 0 and maximum of 0.786). The dendrograms generated by the unweighted pair group method with arithmetic average (UPGMA) did not reflect the regions of Brazil (20 genotypes) or around the world (96 genotypes), where the varieties or lines were selected. Bootstrap resampling shows that genotype identification is viable with 19 loci. The polymorphic markers evaluated are useful to perform varietal identification in a large panel of cotton varieties and may be applied in studies of the species diversity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000700571
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| Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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| Relação: |
10.1590/S0100-204X2015000700007
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| Formato: |
text/html
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| Fonte: |
Pesquisa Agropecuária Brasileira v.50 n.7 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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